Major vault protein promotes locomotor recovery and regeneration after spinal cord injury in adult zebrafish
by Hong-Chao Pan, Jin-Fei Lin, Li-Ping Ma, Yan-Qin Shen, Melitta Schachner
Graphical Abstract
MVP expression levels increased in the spinal cord caudal to the lesion site after spinal cord injury (SCI) in adult zebrafish. Inhibition of MVP by morpholino in vivo impairs recovery of locomotor functions of zebrafish and results in reduced regrowth of axons from the brain. These findings indicate that MVP is an essential regulator of regeneration after SCI and that it may be a potential target for therapy of acute central nervous system lesions.
EJN Blog Supplemental Figures (not peer-reviewed)
The Authors have provided these extra two figures related to this study, specifically for the EJN Blog:
Supplemental Figure 1. MVP expression in normal fish and sham-injury control. (A) MVP expression shows no difference in normal fish when compared to the sham-injured control by Western blot analysis 4 hours after laminectomy. (B) MVP expression shows no difference in uninjured fish when compared to the sham-injured control also by immunofluorescence. MVP (red), DAPI (blue). * indicates the central canal. Scale bar, 100 μm.
Supplemental Figure 2. Immunohistology of MVP expression in the spinal cord. (A-C) MVP expression in the sham-injured control at 11 days and 3 weeks after laminectomy. (D-F) MVP expression in the spinal cord at 11 days after SCI. (G-I) MVP expression in the spinal cord at 3 weeks after SCI. MVP expression 4 mm caudal to the injury site is increased surrounding the central canal at 11 days (D-F) and 3 weeks (G-I) after SCI when compared to the sham-injured control (A-C). MVP (red), DAPI (blue). * indicates the central canal. Scale bar, 200 μm.